![]() A dendrogram generated by incorporating the results of these analyses revealed genetic differences of the strains correlating with their clinical origin. Phenotypic assays for hemolysin activity, serum resistance, and biofilm formation were also performed. ![]() The strains were examined for the presence of genes encoding cholera toxin and toxin-coregulated pilus (TCP), as well as other virulence-associated factors or markers, including hemolysins, repeats-in-toxin (RTX) toxins, Vibrio seventh pandemic islands VSP-1 and VSP-2, and the type III secretion system (TTSS). We studied 18 strains from German and Austrian patients with diarrhea or local infections for their virulence-associated genotype and phenotype to assess their potential for infectivity in anticipation of possible climatic changes that could enhance the transmission of these pathogens. ![]() ![]() These bacteria can cause gastroenteritis and extraintestinal infections, and are transmitted through contaminated food and water. Vibrio cholerae belonging to the non-O1, non-O139 serogroups are present in the coastal waters of Germany and in some German and Austrian lakes. ![]()
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